abstract：:The adhesion molecule CD44 consists of many isoforms of which particularly CD44v7 is of major importance in hematopoietic progenitor cell homing. An increase of progenitor cells in the periphery was observed after treating mice with a CD44v7-specific antibody, concomitant with a substantially augmented marrow-repopula...

journal_title：Journal of leukocyte biology

authors： Christ O,Günthert U,Haas R,Zöller M

更新日期：2001-03-01 00:00:00

abstract：:Colostrum plays an important role in protecting newborn infants against acute gastrointestinal and respiratory infections. IgA antibodies have been considered the major effector component; however, the role of their receptors on colostral phagocytes, especially neutrophils, has not been studied. Here, we demonstrate t...

journal_title：Journal of leukocyte biology

authors： Honorio-França AC,Launay P,Carneiro-Sampaio MM,Monteiro RC

更新日期：2001-02-01 00:00:00

abstract：:In the present study, we investigated the expression of human CD1d antigen on activated mature T cells. Expression of this glycoprotein was found to be highly regulated and dependent on PHA stimulation. Flow cytometry studies using the NOR3.2 antibody, which recognized CD1d under denaturing conditions, showed a clear ...

journal_title：Journal of leukocyte biology

authors： Salamone MC,Rabinovich GA,Mendiguren AK,Salamone GV,Fainboim L

更新日期：2001-02-01 00:00:00

abstract：:The adjuvant effect of gamma-inulin, a strong activator of the alternative complement pathway, is well-known, but its exact mechanism is not revealed yet. Here, we show that macrophages, isolated from the peritoneal cavity of gamma-inulin-injected mice and used as antigen-presenting cells, enhance the proliferation of...

journal_title：Journal of leukocyte biology

authors： Kerekes K,Cooper PD,Prechl J,Józsi M,Bajtay Z,Erdei A

更新日期：2001-01-01 00:00:00

abstract：:Pulmonary alveolar macrophages (AMphis) are incompetent to phagocytose unopsonized Pseudomonas aeruginosa, but ingestion by other macrophage phenotypes (i.e., peritoneal macrophages) occurs efficiently. The purpose of this study was to explore factors that might control such phenotypic differences. Our laboratory has ...

journal_title：Journal of leukocyte biology

authors： Heale JP,Speert DP

更新日期：2001-01-01 00:00:00

abstract：:During inflammation the balance between cell activation and cell death is determined by the tight regulation of multiple intracellular enzyme cascades. Key regulatory steps often involve protein kinases. We show that the prototypical pro-inflammatory molecule, bacterial lipopolysaccharide, activates multiple protein k...

journal_title：Journal of leukocyte biology

authors： Lee J,Mira-Arbibe L,Ulevitch RJ

更新日期：2000-12-01 00:00:00

abstract：:This study was initiated to identify and characterize thyroid fibrosis in a murine model of granulomatous experimental autoimmune thyroiditis (G-EAT) and determine if TGF-beta1 might be involved in fibrosis. G-EAT was induced by transfer of mouse thyroglobulin-sensitized spleen cells activated in vitro with thyroglobu...

journal_title：Journal of leukocyte biology

authors： Chen K,Wei Y,Sharp GC,Braley-Mullen H

更新日期：2000-12-01 00:00:00

abstract：:We have shown that losartan, a selective inhibitor of AT1 receptors for angiotensin II (AII), inhibits the binding of [3H]fMLP to neutrophil receptors (FPR). Here, we analyze, in Wistar rats, the effect of losartan on neutrophil recruitment in the lung triggered by fMLP. We found that i.v. infusion of losartan (0.4-20...

journal_title：Journal of leukocyte biology

authors： Raiden S,Pereyra Y,Nahmod V,Alvarez C,Castello L,Giordano M,Geffner J

更新日期：2000-11-01 00:00:00

abstract：:Fluid-phase endocytosis is stimulated by H-ras-linked growth factor receptors and this stimulation requires activation of rab5. We utilized a GFP-rab5a:wt fusion protein to monitor GFP-rab5a:wt activation in living fibroblasts and in J774 macrophages. Control CHO cells that expressed GFP-rab5a:wt were cultured in seru...

journal_title：Journal of leukocyte biology

authors： Roberts RL,Barbieri MA,Ullrich J,Stahl PD

更新日期：2000-11-01 00:00:00

abstract：:The in vitro studies have proposed that human Th1 cells favor expression of CXCR3 or CCR5, whereas Th2 cells favor CCR3 and CCR4. In this study, the in vivo relevance of expression of these chemokine receptors on Th cells was investigated in patients with atopic dermatitis (AD) as the Th2-dominated disorder and nonato...

journal_title：Journal of leukocyte biology

authors： Yamamoto J,Adachi Y,Onoue Y,Adachi YS,Okabe Y,Itazawa T,Toyoda M,Seki T,Morohashi M,Matsushima K,Miyawaki T

更新日期：2000-10-01 00:00:00

abstract：:Osteopontin is an RGD-containing bone matrix protein with cytokine-like functions that is associated with early stages of Th1-mediated diseases. Although the function of osteopontin in these responses is unknown, it is expressed by activated T cells and macrophages and can costimulate T cell proliferation. Studies hav...

journal_title：Journal of leukocyte biology

authors： O'Regan AW,Hayden JM,Berman JS

更新日期：2000-10-01 00:00:00

abstract：:Human immunodeficiency virus type-1 (HIV-1)-associated dementia (HAD) is a neurodegenerative disease characterized by HIV infection and replication in brain tissue. HIV-1-infected monocytes overexpress inflammatory molecules that facilitate their entry into the brain. Prostanoids are lipid mediators of inflammation th...

journal_title：Journal of leukocyte biology

authors： Pereira CF,Boven LA,Middel J,Verhoef J,Nottet HS

更新日期：2000-09-01 00:00:00

abstract：:During highly active antiretroviral therapy (HAART), HIV-1 can still persist in circulating, resting CD4+ T lymphocytes, lymph node mononuclear cells, and seminal cells of patients despite sustained suppression of plasma viremia to undetectable levels. Sanctuary sites where antiretroviral drug penetration is not optim...

journal_title：Journal of leukocyte biology

authors： Crowe SM,Sonza S

更新日期：2000-09-01 00:00:00

abstract：:Neutrophil-induced damage to the protective epithelium has been implicated in mucosal disorders associated with hypoxia, and such damage may be initiated by epithelial-derived chemokines. Because chemokines can bind to membrane proteoglycans, we hypothesized that chemokines may associate with epithelial surfaces and a...

journal_title：Journal of leukocyte biology

authors： Furuta GT,Dzus AL,Taylor CT,Colgan SP

更新日期：2000-08-01 00:00:00

abstract：:Underlying acquired immunity to the fungus Candida albicans is usually present in adult immunocompetent individuals and is presumed to prevent mucosal colonization progressing to symptomatic infection. Exploration of immunological events leading to Candida resistance or susceptibility has indicated the central role of...

journal_title：Journal of leukocyte biology

authors： Romani L

更新日期：2000-08-01 00:00:00

abstract：:Interleukin-1beta is a secreted protein that accumulates in the cytosol as an inactive precursor (pIL-1beta) before processing and release of biologically active protein. To understand the impact of this property on IL-1beta production, we examined the intracellular stability of pIL-1beta in lipopolysaccharide (LPS)-s...

journal_title：Journal of leukocyte biology

authors： Moors MA,Mizel SB

更新日期：2000-07-01 00:00:00

abstract：:Neutrophil activation is a multistep process. In vitro activation of neutrophils with semiphysiological activators is optimal only after preactivation or priming with cytokines, chemotaxins, and/or bacterial products. Until now, no antibodies have been developed that can distinguish between resting and (cytokine) prim...

journal_title：Journal of leukocyte biology

authors： Koenderman L,Kanters D,Maesen B,Raaijmakers J,Lammers JW,de Kruif J,Logtenberg T

更新日期：2000-07-01 00:00:00

abstract：:Virulent Legionella pneumophila replicate readily in thioglycollate-elicited peritoneal macrophages from genetically permissive A/J mice, but avirulent L. pneumophila do not. The production of cytokines by macrophages infected with L. pneumophila has been studied, but the correlation of bacterial virulence with immune...

journal_title：Journal of leukocyte biology

authors： McHugh SL,Yamamoto Y,Klein TW,Friedman H

更新日期：2000-06-01 00:00:00

abstract：:To assess the effect of hypoxemia on the responses of polymorphonuclear neutrophils (PMN) during an inflammatory response, rats were maintained in a low F1O2 atmosphere (9% O2) or room air for 12 h before intrathoracic injection of carrageenin or intradermal injections of agonists. After 4 h, hypoxemic rats had 50% mo...

journal_title：Journal of leukocyte biology

authors： Tremblay PB,Macari DM,Martel D,du Souich P,Barja-Fidalgo C,Marleau S

更新日期：2000-06-01 00:00:00

abstract：:The lineage-specific Charcot-Leyden crystal (CLC) protein is found in human eosinophils and basophils where it comprises 7-10% of the cellular protein content. Previous work from our laboratory has identified the motif GGAGA[A/G] as a powerful enhancer of gene transcription ill two eosinophil ribonuclease genes. To ev...

journal_title：Journal of leukocyte biology

authors： Dyer KD,Rosenberg HF

更新日期：2000-05-01 00:00:00

abstract：:Group B streptococcal (GBS) infections are associated with high morbidity and mortality. The molecular pathways mediating the pathophysiological events in GBS infection are not fully delineated. Cyclooxygenases (COX) are the enzymes that convert arachidonate to active eicosanoids. To identify the effects of GBS on eic...

journal_title：Journal of leukocyte biology

authors： Maloney CG,Thompson SD,Hill HR,Bohnsack JF,McIntyre TM,Zimmerman GA

更新日期：2000-05-01 00:00:00

abstract：:Hematopoietic cell differentiation takes place in phenotypically recognizable stages characterized by morphology as well as by the expression of enzymes and surface markers. It is recognized that differentiation results from an interaction of environmental cues, such as cytokines and hormones, with internal cellular p...

journal_title：Journal of leukocyte biology

authors： Chen F,Rao J,Studzinski GP

更新日期：2000-04-01 00:00:00

abstract：:A continuing theme of work done in our laboratory involves regulation of adaptive immune response by innate cells, in general, and immuneregulation by natural killer (NK) and NKT cells, in particular. Studies include work with the lung and the eye. In addition to immune surveillance of tumor cells, the NK cell is ofte...

journal_title：Journal of leukocyte biology

authors： Stein-Streilein J,Sonoda KH,Faunce D,Zhang-Hoover J

更新日期：2000-04-01 00:00:00

abstract：:Tumor necrosis factor (TNF)-stimulated gene 14 (TSG-14, also termed PTX3) encodes a secreted glycoprotein whose carboxy-terminal half shares sequence similarity with the pentraxin family of acute phase proteins (C-reactive protein and serum amyloid P component). We compared TSG-14 mRNA expression in cultures of murine...

journal_title：Journal of leukocyte biology

authors： Goodman AR,Levy DE,Reis LF,Vilcek J

更新日期：2000-03-01 00:00:00

abstract：:Macrophages play an important role in effector mechanisms of various chronic inflammatory diseases. We studied the effect of gluten, the agent inducing celiac disease, and other food antigens on the activation of macrophages. Nitric oxide (NO) and cytokine production were followed as markers of activation, using cultu...

journal_title：Journal of leukocyte biology

authors： Tucková L,Flegelová Z,Tlaskalová-Hogenová H,Zídek Z

更新日期：2000-03-01 00:00:00

abstract：:Neutrophils can attach to E-selectin under flow. Proposed ligands for E-selectin carry SLe(x)-type glycans. The leukocyte beta2 integrins are glycosylated with SLe(x). Thus, we speculated that beta2 integrins could support attachment to E-selectin. To test this hypothesis, we coated 10-microm-diameter microspheres wit...

journal_title：Journal of leukocyte biology

authors： Crutchfield KL,Shinde Patil VR,Campbell CJ,Parkos CA,Allport JR,Goetz DJ

更新日期：2000-02-01 00:00:00

abstract：:Previously we had determined that neonatal neutrophils had decreased interaction with monolayers expressing P-selectin compared to adult cells. In this study we examined the function of neonatal P-selectin glycoprotein ligand-1 (PSGL-1). A rabbit polyclonal antibody directed against the amino terminus of human PSGL-1 ...

journal_title：Journal of leukocyte biology

authors： Tcharmtchi MH,Smith CW,Mariscalco MM

更新日期：2000-01-01 00:00:00

abstract：:Murine peritoneal exudate cells (PEC) pre-exposed to bacterial lipopolysaccharide (LPS) show augmented nitric oxide (NO) production by LPS restimulation, in contrast to LPS tolerance with reduced production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6). Significant amounts of interferon-gamma (IF...

journal_title：Journal of leukocyte biology

authors： Tominaga K,Saito S,Matsuura M,Funatogawa K,Matsumura H,Nakano M

更新日期：1999-12-01 00:00:00

abstract：:Pulmonary macrophages (Mphi) increase in tissue and bronchoalveolar lavage (BAL) fluid during inflammation caused by bleomycin (BLM). This study demonstrates that increasing numbers of exudate Mphi in BLM lung injury exhibit dendritic cell (DC) features. After the intratracheal administration of BLM (0.075 U), adheren...

journal_title：Journal of leukocyte biology

authors： Tager AM,Luster AD,Leary CP,Sakamoto H,Zhao LH,Preffer F,Kradin RL

更新日期：1999-12-01 00:00:00

abstract：:CD163 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. This study describes aspects of the tissue distribution, the regulation of expression, and signal transduction after cross-linking of this receptor at the cell surface of macrophages. CD163 showed an exclusive expression on resident ...

journal_title：Journal of leukocyte biology

authors： Van den Heuvel MM,Tensen CP,van As JH,Van den Berg TK,Fluitsma DM,Dijkstra CD,Döpp EA,Droste A,Van Gaalen FA,Sorg C,Högger P,Beelen RH

更新日期：1999-11-01 00:00:00

abstract：:In vivo, dendritic cells (DC) form a network comprising different populations. In particular, Langerhans cells (LC) appear as a unique population of cells dependent on transforming growth factor beta(TGF-beta) for its development. In this study, we show that endogenous TGF-beta is required for the development of both ...

journal_title：Journal of leukocyte biology

authors： Caux C,Massacrier C,Dubois B,Valladeau J,Dezutter-Dambuyant C,Durand I,Schmitt D,Saeland S

更新日期：1999-11-01 00:00:00

abstract：:We have investigated the role of the purinergic P2X7 receptor in the formation of multinucleated giant cells in human monocyte/macrophage cultures stimulated with either concanavalin A or phytohemagglutinin. Macrophage fusion can be blocked by a P2X7-selective pharmacological antagonist or by a mAb directed against th...

journal_title：Journal of leukocyte biology

authors： Di Virgilio F,Falzoni S,Chiozzi P,Sanz JM,Ferrari D,Buell GN

更新日期：1999-11-01 00:00:00

abstract：:Azurocidin is a multifunctional endotoxin-binding serine protease homolog synthesized during the promyelocytic stage of neutrophil development. To characterize the biosynthesis and processing of azurocidin, cDNA encoding human preproazurocidin was stably transfected to the rat basophilic leukemia cell line RBL-1 and t...

journal_title：Journal of leukocyte biology

authors： Lindmark A,Garwicz D,Rasmussen PB,Flodgaard H,Gullberg U

更新日期：1999-10-01 00:00:00

abstract：:Studies of the metabolism and function of the amyloid precursor protein (APP) and its proteolytic fragment A beta in cultured cells, transgenic mice, and post-mortem brain tissue have advanced our understanding of Alzheimer disease (AD). However, the molecular pathogenesis of the disease is still not clear, and we are...

journal_title：Journal of leukocyte biology

authors： Li QX,Fuller SJ,Beyreuther K,Masters CL

更新日期：1999-10-01 00:00:00

abstract：:Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultur...

journal_title：Journal of leukocyte biology

authors： Vecchi A,Massimiliano L,Ramponi S,Luini W,Bernasconi S,Bonecchi R,Allavena P,Parmentier M,Mantovani A,Sozzani S

更新日期：1999-09-01 00:00:00

abstract：:Vascular occlusion is the main cause of the morbidity and mortality observed in patients with sickle cell disease (SCD). Increasing evidence indicates that (activated) neutrophils could play an important role in the initiation and propagation of vaso-occlusive processes in SCD. In this study, the activation state of n...

journal_title：Journal of leukocyte biology

authors： Lard LR,Mul FP,de Haas M,Roos D,Duits AJ

更新日期：1999-09-01 00:00:00

abstract：:Both humoral and cellular immune responses are inducible by inoculation of naked plasmid DNA encoding a polypeptide antigen. This new vaccination protocol, known as genetic immunization, has been used to initiate protective immunity against a variety of infectious pathogens and tumors in experimental animals. Dendriti...

journal_title：Journal of leukocyte biology

authors： Takashima A,Morita A

更新日期：1999-08-01 00:00:00

abstract：:There is growing evidence that, in addition to their role as initiators of immune responses, dendritic cells (DC) can exhibit tolerogenic properties. Immature DC deficient in cell surface costimulatory/accessory molecules can prolong organ and pancreatic islet allograft survival, whereas in vitro manipulation of DC by...

journal_title：Journal of leukocyte biology

authors： Lu L,Lee WC,Takayama T,Qian S,Gambotto A,Robbins PD,Thomson AW

更新日期：1999-08-01 00:00:00

abstract：:We show that interleukin-18 is constitutively produced by dendritic cells; synthesis and secretion are poorly affected by maturative stimuli. Challenge of dendritic cells with autologous anti-tetanus toxoid T lymphocytes results in a secretory switch, with induction of secretion of biologically active interleukin-18 a...

journal_title：Journal of leukocyte biology

authors： Gardella S,Andrei C,Costigliolo S,Poggi A,Zocchi MR,Rubartelli A

更新日期：1999-08-01 00:00:00

abstract：:Nitric oxide (NO) is a well-documented effector molecule in rodent phagocytes but its synthesis in human neutrophils has been controversial. In this study, NO production in human neutrophils activated by chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) was measured in the presence of L-arginine (L-Ar...

journal_title：Journal of leukocyte biology

authors： Holm P,Kankaanranta H,Oja SS,Knowles RG,Moilanen E

更新日期：1999-07-01 00:00:00